Forming of the ethnographic collection began with the very foundation of the museum, when the entry into fund of one of the more valuable items of the Serbian urban costume, female caraco, registered as the wedding libade, was noted. With the organization of the department for ethnology (1965) began the systematic study of the traditional culture and forming of the collections which included objects from the territory of Kruševac, but also from a wider anthropogeographic whole of a part of Temnić and West Pomoravlje, Rasina, villages under mountain Kopaonik and the area of Župa of Aleksandrovac. The collection reflected all the characteristics of the ethnical structure of the area of Kruševac, where after the liberation (1833), besides the small number of natives, were mostly the immigrants from Kosovo and Metohija, mixed with the later Dinaric immigrants.

The objects in the ethnological collection evidence on everyday life of the population in this area, closer and further in the past and in various social and economic conditions. They are classified and deposited by the kind of material, so that over time the collections and sub-collections could be formed. The ethnological collection, with about 6000 registered items, is nowadays assorted into items belonging to the rural and the urban culture.